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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP887686Yeast1mgQuote
EP887686E. coli1mgQuote
BP887686Baculovirus200ugQuote
MP887686Mammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Uroleucon erigeronensis
UniProt IDQ9EVH6
Gene NameleuA
Protein Name2-isopropylmalate synthase
Region Expressed1-503
Expression Tag6xHis
Purity>90%
AA SequenceDGEQALQASLSVEEKLXIALCLEKTGVDIMEVGFPISSPGDFKSVQTISKKIKNSQICSL ARCIEKDIDIAGEAMSVCDFFRIHIFLATSRLHMKSKLRKNFDEIIDMAIFSVKRALRYT DDVQFSCEDASRTTIDNLCRIVEKLINSGVKTINIPDTVGYSIPNELSFIIKNLFQRVPN IDKAVISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVREK NLGVYTNINHKEIYRTSQMISRICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEI IDPVSIGLKNVQLNLTSRSGRAAVKYYMNEMGYKDSDYNIDELYSDFLKLADKKGQVFDY DLESLAFISKQQDELEYFCLKFFSVQSISNGLSTASVKLLCGKKVYTESSTTSNGPVDAI YQALNRITHFPITLQKFQLIAKGKGKDALGQVDILVEYKKRKFHGVGLATDIIESSAKAM INVLNNIWKAKQVNKKLKILKKK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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