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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP884169Yeast1mgQuote
EP884169E. coli1mgQuote
BP884169Baculovirus200ugQuote
MP884169Mammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Uroleucon rurale
UniProt IDQ9EVE3
Gene NameleuA
Protein Name2-isopropylmalate synthase
Region Expressed1-437
Expression Tag6xHis
Purity>90%
AA SequenceEKLQIALSLEKIGVDIMEVGFPISSPGDFKSVQTISKNIKNSRICSLARCLEKDIDAAGE AMSVCDNFRIHIFLATSTLHIESKLRKNFNEIIEMAIFSVKKALRYTNDVEFSCEDASRT NIDNLCRIVEKLIKSGVKTINIPDTVGYSTPDELTFIIKNLFERVPNIHKSIISVHCHDD LGMAVGNSIAAIQAGARQIEGTINGIGERAGNTALEEVIMAIKVRKNILGVSTNINHKEI YRTSQMISRICNMPIPSNKAIVGSNAFSHSSGIHQDGVLKNRENYEIMDPRSIGLKKVKL NLTSRSGRAAVKYYMSEMGYKDSDYNIDELYTDFLKLADKKGQVFDYDLESLAFINKQQD ELEYFRLQFFSVQSISNNLSTASVKLLCGNKTYTESSTTSNGPVDAIYQALNRITHFPII LQKFQLIAKGKGKDALG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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