Request QuoteCatalog Number: xP864606VEXSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP864606VEXYeast1mgQuote
EP864606VEXE. coli1mgQuote
BP864606VEXBaculovirus200ugQuote
MP864606VEXMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
UniProt IDQ9KP83
Gene NameleuA; Locus:VC_2490
Protein Name2-isopropylmalate synthase
Region Expressed1-516
Expression Tag6xHis
Purity>90%
AA SequenceMNNQVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDIIEAGFPVSSPGDFESVQT IAKNIKNSRVCALSRAVAKDIDAAAEALKVAEAFRIHTFISTSTIHVQDKLRRSYDDVVA MAVKAVKHARQYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGARTINIPDTVGYTVPSEF GGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAIQAGARQVEGTINGIGERAGNCA LEEIAMIIKTRQELLGVTTGIKHDEISRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIH QDGMLKNKNTYEIMTPESIGLKNQALNLTSRSGRAAVKSHMDSMGYNENEYNLDALYEDF LKLADRKGQVFDYDLEALMHFSNLREEDDFYKLNYLSVQSGSVMATTSIKLLCGGEEKCE AAVGNGPVDALYQCIYRITGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS TDIVEASGQALLHVINSIHRADQIAQIKQKKSVATV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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