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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP846122LGUYeast1mgQuote
EP846122LGUE. coli1mgQuote
BP846122LGUBaculovirus200ugQuote
MP846122LGUMammalian Cell200ugQuote

Protein Information

SpeciesListeria innocua serovar 6a (strain CLIP 11262)
UniProt IDQ92A28
Gene NameleuA; Locus:lin2094
Protein Name2-isopropylmalate synthase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMKKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAI AKAIKHCSVTGLARCVEADIDRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLAS IKHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFG QLFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGARRVEGTINGIGERAGNTAL EEVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQ DGVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFNTRMEEMGYTLSEQEQKDAFKRFK QLADAKKDVTEEDLHALILGQSSESHDAFELKHLQVQYVTGGVQGAIVRIEERDGALIED AATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDAQAEVHVVIKDDNGTEFHGIGI DFDVLTASAKAYLQASGKSKSTSKQADFEEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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