Request QuoteCatalog Number: xP756148RDRSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP756148RDRYeast1mgQuote
EP756148RDRE. coli1mgQuote
BP756148RDRBaculovirus200ugQuote
MP756148RDRMammalian Cell200ugQuote

Protein Information

SpeciesRhodopirellula baltica (strain SH1)
UniProt IDQ7UI51
Gene NameleuA; Locus:RB12756
Protein Name2-isopropylmalate synthase
Region Expressed1-527
Expression Tag6xHis
Purity>90%
AA SequenceMNQSAEAPSSENVATRQIRIFDTTLRDGEQSPGASMNLAEKLEVASALVDLGVDIIEAGF PIASPGDFESVRQIATTIRGATICGLARCAEKDIDRAWEALKTAPQARIHVFLATSAIHR EFKLRMTPDEIVERAVAGVRRAASHCDDVEFSPEDACRTEHDFLCRVVEAAIDAGATTIN VPDTVGYTTPGEIYDRFKMLRDRVPNMDKAVLSTHCHDDLGMAVANSLAAVDAGAGQIEC TINGIGERAGNAALEELVMAMKTRQDFYHCQTNINSKRLVPVSRLVSKTTGINVQRNKAI VGRNAFAHESGIHQDGMLKERTTYEIMSPEEVGFTKTDLVLGKHSGRAALADRAKQLGMT LTGEQLQEVFEAFKELADKKKEIYDGDIVALVQQKISETVAPEWTLVDYEVTSGKNQTPN VRVTLRRDEQDITEQVAQGDGPIDAAFWAVEKITGIQVVCKDFRVRSATLGRDAIGEVNL EVEHEGKTYRGTGVSTDSVESTILAMLNAINRIVAGIASDPGELPQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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