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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP756120SSLYeast1mgQuote
EP756120SSLE. coli1mgQuote
BP756120SSLBaculovirus200ugQuote
MP756120SSLMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain WH8102)
UniProt IDQ7U892
Gene NameleuA; Locus:SYNW0730
Protein Name2-isopropylmalate synthase
Region Expressed1-540
Expression Tag6xHis
Purity>90%
AA SequenceMAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASAGDFAA VQRIAQQVGGENGPIICGLARASQRDIKACAEAVSPAPRRRIHTFIATSDIHLEHKLRKS RADVLGIVPEMVSYARSLMDDIEFSCEDAGRSDPEFLYEVIEAAIAAGATTINIPDTVGY TTPSEFGDLIAGINRYVPNIGEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE RAGNASLEELVMALHVRRRYFNPFFGRDQDSPTPLSAVRTEELTKTSRLVSNLTGMVVQP NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNRISLGKLSGRSAVRARLEE LGYDLTREDLDEAFARFKDLADRKREITDRDLEAIVSEQVQQPEARYQLQLVQVSCGTRL KPTATVALSEENGPDQTVSAVGTGPVDAVCRALNQLAGVPNELIEFSVKSVTEGIDAMGE VTIRLRRDGSLYSGHAADTDVVVAAAMAFINALNRLVAAQEHQPLHPQRDAVVLDARPTL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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