Request QuoteCatalog Number: xP766894BHMSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP766894BHMYeast1mgQuote
EP766894BHME. coli1mgQuote
BP766894BHMBaculovirus200ugQuote
MP766894BHMMammalian Cell200ugQuote

Protein Information

SpeciesBlochmannia floridanus
UniProt IDQ7VQJ6
Gene NameleuA; Locus:Bfl133
Protein Name2-isopropylmalate synthase
Region Expressed1-519
Expression Tag6xHis
Purity>90%
AA SequenceMHQQVIIFDTTLRDGEQSLQTSLSVKEKLQIAFALEKLGVDVMEVGFPISSPGDFESVHT IAQNIQNSCVCGLARCLEKDIDIAAEALKSAKKFRIHIFLPTSAIHIQSKLRKNFDQIID MTTHSIRYARKYTDDVEFSCEDAGRTSIDNLCRIVEIAIKSGARTINIPDTVGYTIPNQF SEIIYSLYKKVPIIDQAIISVHCHDDLGMAVGNSISAIQAGARQIEGTINGIGERAGNTA LEEIIMAIKIRHDLLHVYTNIHHHEIYQTSQVVSQLCNMPIPANKAIVGSNAFSHSSGIH QDGVLKNKKNYEIMNPKNIGLKKVQLNLTSRSGRAAVKYHMEKMGYQEHDYNIDELYKVF LKLADQKGRVFNYELEALAFTKYSQEYLEYFALESFHIHSTSSALSNAIINLYCGKEKKI HSCSTTGRGPIDAAYKALTLISKLPIKLEKYQLHSKKYEYNILGQVNIIVSYQGRHFHGI GSNIDIIKASIIAMIQALNNIWQTKQITINHKNTQHIKK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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