Request QuoteCatalog Number: xP763481HCOSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP763481HCOYeast1mgQuote
EP763481HCOE. coli1mgQuote
BP763481HCOBaculovirus200ugQuote
MP763481HCOMammalian Cell200ugQuote

Protein Information

SpeciesHelicobacter hepaticus (strain ATCC 51449 / 3B1)
UniProt IDQ7VH30
Gene NameleuA; Locus:HH_1137
Protein Name2-isopropylmalate synthase
Region Expressed1-558
Expression Tag6xHis
Purity>90%
AA SequenceMSYTKYQRGYFMPPTPSLEWSKKEYIAKAPIWCSVDLRDGNQALITPMSLEEKITFFKLL LKVGFKEIEVGFPAASETEYRFLRTLIEEDLIPDDVSVQVLTQAREHIIKKTFESLEGCK NAIVHVYNSTSYAQREQVFRKSKDEIKHIACEGAICLKESAAATKGNFRFEYSPESFSGT EVDYALEVCNAVIDIFEPTLEKKLIINLPVTVEMSLPHIYASQVEYMSKHLKNRENVLIS LHPHNDRGCAIADAELGLLAGADRIEGTLFGNGERTGNVDIITLAMNMHSHGVDPKLDFS NIPSICDVYEKVTKMQVYERQPYSGKLVFAAFSGSHQDAIAKGMAYHKEHNLSTWSVPYL PIDPKDVGRVYESDVIRINSQSGKGGIAYILHHHYGVNLPPLFREEFSYYVKNISDKAHK ELSPDEICEIFQNDFVNLNNTLCVQDFHFTHIDSKPSQEFNVKLHILYAKENSKTQQEIT GKGNGRLDSIANALREHLNLDFEIIDYSEHSLKKGSTSQAVSYVQIESKGKKCFGVGIDN DIITASIYGLVSALNRII
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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