Request QuoteCatalog Number: xP742292GCISize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP742292GCIYeast1mgQuote
EP742292GCIE. coli1mgQuote
BP742292GCIBaculovirus200ugQuote
MP742292GCIMammalian Cell200ugQuote

Protein Information

SpeciesGloeobacter violaceus (strain PCC 7421)
UniProt IDQ7NI93
Gene NameleuA; Locus:gll2290
Protein Name2-isopropylmalate synthase
Region Expressed1-538
Expression Tag6xHis
Purity>90%
AA SequenceMFQDRLIIFDTTLRDGEQSPGATLNADEKVEIARQLARLGVDVIEAGFAYASPGDFEAVE RVARTVGTEDGPVICSLARAIRSDIQAAAEAIRPAARGRIHTFISTSDIHLEHQLRKSRA EVLAIAAEMVAFAKGFVDDVEFSPMDAGRSAPEYLYRVLEAAIAAGATTVNIPDTVGYLT PAEFGGLIRGITQNVRGIERAVISVHCHNDLGLAVANSLAAIENGARQIECTVNGIGERA GNCSLEEIVMALHVRRQFFNPIFGRPADSTVPLSTIDTRQIYKSSRLVSHLTGMLVQPNK AIVGANAFAHESGIHQDGVLKNRLTYEIMDAETVGVNENRIVLGKHSGRNAFRTRLVELG YELGDADLNRAFLRFKELADKKKTVSDWDIEAVISDEIRLIPEAYRLEQVQVSCGEPGLP TATVRLTGPDGVERVDAAVGTGPVDAVYKAINRLIELPNELIEFSVQSVTAGIDAMGEVT IRVRQDGRTFSGHAANTDIIVASARAYLNALNKLHFALAHPTHSGGALAHPDAAAQKL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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