Request QuoteCatalog Number: xP343363SSQSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP343363SSQYeast1mgQuote
EP343363SSQE. coli1mgQuote
BP343363SSQBaculovirus200ugQuote
MP343363SSQMammalian Cell200ugQuote

Protein Information

SpeciesSynechocystis sp. (strain PCC 6803 / Kazusa)
UniProt IDP48576
Gene NameleuA; Locus:slr0186
Protein Name2-isopropylmalate synthase
Region Expressed1-533
Expression Tag6xHis
Purity>90%
AA SequenceMNSPVDRILIFDTTLRDGEQSPGATLTVEEKLSIARALARLGVDIIEAGFPFASPGDFEA VQKIAQTVGTENGPVICGLARATQKDIKAAAEALKPAAKHRIHTFLATSDIHLEHKLKKT RAEVLAIVPEMVAYAKSLVNDIEFSPEDAGRSDPEFLYQVLEAAISAGATTINIPDTVGY TTPAEYGALIKGIADNVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE RAGNAALEELVMALHVRRSYFNPFLGRPADSTAPLTNIDTKHIYATSRLVSELTGMMVQP NKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAESIGLTNNQIVLGKLSGRNAFGTRLKE LGFDLSDTELNNAFIRFKEVADKRKEITDWDLEAIVNDEIRQPPELFRLERVQVSCGEPS VPTATLTIRTPAGPEETAVAIGTGPVDAVYKAINQIVQLPNELLEYSVTSVTEGIDALGK VSVRLRHNGVIYTGYAANTDIIVASARAYLGALNRLYAALEKSREHPPVVASL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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