Request QuoteCatalog Number: xP341772NHRSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP341772NHRYeast1mgQuote
EP341772NHRE. coli1mgQuote
BP341772NHRBaculovirus200ugQuote
MP341772NHRMammalian Cell200ugQuote

Protein Information

SpeciesNostoc sp. (strain PCC 7120 / UTEX 2576)
UniProt IDP48575
Gene NameleuA; Locus:alr4840
Protein Name2-isopropylmalate synthase
Region Expressed1-531
Expression Tag6xHis
Purity>90%
AA SequenceMTTKPEKIIIFDTTLRDGEQCPGATLNIDEKLAIAKQLARLGVDIIEAGFAFASPGDFEA VHKIAQTVGTQSGPVICSLARARHDDIKAAAEAIKPAAKGRIHTFIATSDIHLQYKLKKT RPEVIAIAEEMVAYAKSFTDDVEFSPEDAGRSDPEFLYQVLERAIAAGATTINIPDTVGY TTPSEFGAIIKGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE RAGNAALEELVMAMHVRRQYFNPFLGRHPDSEEALTNIDTKQIYKTSRLVSNLTGMLVQP NKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQLIGLTDNQIVLGKHSGRNAFRTRLKE LGFELSETELNKAFVKFKEVADKKKEISDWDLEAIVNDEIQQAPDLFRVELVQVSCGSNA RPTATVTLRTPDGEELTDAAIGTGPVDAVYKAINRVVNVPNQLIEFSVQSVTAGIDAIGE VTIRLRYESRVFSGHAANTDIIVASAQAYVNALNRLYASLQTQDKQTEVTA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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