Request QuoteCatalog Number: xP520593SWGSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520593SWGYeast1mgQuote
EP520593SWGE. coli1mgQuote
BP520593SWGBaculovirus200ugQuote
MP520593SWGMammalian Cell200ugQuote

Protein Information

SpeciesSalinibacter ruber (strain M8)
UniProt IDD5HB86
Gene NameleuA; Locus:SRM_02370
Protein Name2-isopropylmalate synthase
Region Expressed1-546
Expression Tag6xHis
Purity>90%
AA SequenceMSDSITIFDTTLRDGEQAPGASMTVPEKVHIAHKLADLNVDVIEAGFPISSPAQTEAVTR IATEVDGPVTCALARTKEDDIDAAGEALADGTDTRLHTFIATSDVHIEAKFDKLGNTMAE KREAIIQRAVRAIEQALTYTDNVEFSAEDAGRTDPEFLCEVVQAAAEAGATTINIPDTTG YCAPSEYTDLLETVVDCLPDPDAVTLSTHCHDDLGLATANTLAGIRAGARQVECTINGIG ERAGNAALEEIVMALTVRADAFDVTADVHTEHLTPTSQTVSAATGFPVQPNKAIVGSNAF SHEAGIHQHGVLEERTTYEIMSATDVGQDAEQIRLGRHSGRHGLFNRLEAMGYAVPEGHR DALYDRFLDLADRKKEVFEEDLEQMMNDFGGDAVAAATGLPDNGVALNGGTPAYRLDQFS VHLSSDDEAKVSVRLQRDDGSAREEQATGEGPVDALYRALDHAVDAPHTLVDYSIRSISE GADAQGEVEVTIRYGENQFAGTARNTDVIRASAEAYVDALNRLVAAQEHAESVEFVQNGI MHTYGE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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