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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515023FORYeast1mgQuote
EP515023FORE. coli1mgQuote
BP515023FORBaculovirus200ugQuote
MP515023FORMammalian Cell200ugQuote

Protein Information

SpeciesStreptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100)
UniProt IDD2ATJ4
Gene NameleuA; Locus:Sros_3794
Protein Name2-isopropylmalate synthase
Region Expressed1-579
Expression Tag6xHis
Purity>90%
AA SequenceMKTTTRQTGRYGAFTPVPLPGRTWPNNIIGSAPRWLSTDLRDGNQSLATPMSPDRKLAMF ELLVSMGYKEIEVGFPVASQDDFDFLRVLIEQERIPEDVRISVLVQARDELIRRTVESLE GAPRATIHLYNATSPLFRRVVFGMSRNECKDLAVQGTRLMMKYAEKTLGDCDLGFQYSPE LFSDTELDFSLEVCEAVMDVWQPEPGRGIILNFPTTVERSLPNVFADQIEWLSRNLSRRE HVCLSIHPHNDRGTAVASAELALLAGAERIEGCLFGNGERAGNVCLVTLGLNMFTHGVDP GIDFSDINEIRRTVERCNGLAVHPRHPYGGDLVYTSFSGSHQDAIKKGFDALEREAAHRG AAVGDLPWEMPYLPLDPEDVGRTYEAVVRINSQSGKGGVAYVMSAWHGLNLPRDLQIDFA HVVQSQADAEGGEITPDRVKRLFEREYLSSSDLPVPLASGTELVTTSLHIDGERFDVGAD RADTVQAVRATLTRWGFDVRAVHRTGIAGQDRGPDADVAVYAECRVEGRVSWGVGIDRDI EAASLAAVRSAVIRARLKRLPARGGEATGIVAPLVAAGR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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