Request QuoteCatalog Number: xP505421DJPSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505421DJPYeast1mgQuote
EP505421DJPE. coli1mgQuote
BP505421DJPBaculovirus200ugQuote
MP505421DJPMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio magneticus (strain ATCC 700980 / DSM 13731 / RS-1)
UniProt IDC4XPA8
Gene NameleuA; Locus:DMR_15980
Protein Name2-isopropylmalate synthase
Region Expressed1-513
Expression Tag6xHis
Purity>90%
AA SequenceMSDDNRVYIFDTTLRDGEQSPGATMTREEKVRMARQLETLGVDIIEAGFPAASEGDFQAV SAIAAAVKTPVVAALCRALASDIDRGFEAIKGAQRRRIHTFLATSELHMQHKLNKTPTQV LDMIEAAVSHAASKGVEVQFSAEDASRSEPAFLVAACERAINAGATILNIPDTVGYAQPA EFAELIRHLMTTVRGAGGVTFAVHCHNDLGLAVANTLAALHAGARQAEVTLSGIGERAGN ASLEQVVMGLNTRPNYYNLTTGIVTEELFPSCRRLSGIIGQPIPPYAPIMGRNAFAHESG IHQHGVLKDRRTYEIMTAESIGRKGAVVVLGKHSGRHALDAKVKELGYALNDEELLVVFV AVKQLADRKQRILDEDIEALILEKVLRRPDRYALQFLSVHCGNVELAPFAVVEMQVEGQT VRHYSAGSGPVDAVFNAVCQAVGRKPDLEEYQINAITGGTDAQGEVTVRIKDGTATTVGR GVHDDVIMASTLAFINALNRLAKKEEERTCPQL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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