Request QuoteCatalog Number: xP504939TOQSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP504939TOQYeast1mgQuote
EP504939TOQE. coli1mgQuote
BP504939TOQBaculovirus200ugQuote
MP504939TOQMammalian Cell200ugQuote

Protein Information

SpeciesTolumonas auensis (strain DSM 9187 / TA4)
UniProt IDC4LAV7
Gene NameleuA; Locus:Tola_0682
Protein Name2-isopropylmalate synthase
Region Expressed1-521
Expression Tag6xHis
Purity>90%
AA SequenceMSDRVIIFDTTLRDGEQALPASLTVREKLQIALALERLGVDVMEVGFPVSSPGDFESVQT IARQIKNSRVCALSRALQKDIDAAAEALKVADQFRIHTFLATSTIHVESKLKKSFEDVLE MGINAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAINAGARTINIPDTVGYTIPSEF GGIIHNLFNRVPNIDKAVISVHCHDDLGLSVANSISAVQMGARQVECTINGIGERAGNTS LEEVAMILRTRQDLIGVHTNIKHQEIHRTSQLVSQLCNMPVQPNKAIVGANAFSHSSGIH QDGVLKAKNTYEIITPESVGLNQNTLNLTSRSGRHVIKHRLALLGYPEGSYDLDQIYTSF LQLADKKGQVFDYDLEALLFFSQIQEEPEHFKLNYLSVQSGGSIMATASVRMKVGDEEVT EAATGNGPVDAVYQCINRITGYEINISKYDLKSKGIGKDALGQVDIVAEFKGRKFHGMGL ATDIVESSAQALVHVINNIYRALQVAEHKERFAQKTVMETL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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