Request QuoteCatalog Number: xP488841DULSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP488841DULYeast1mgQuote
EP488841DULE. coli1mgQuote
BP488841DULBaculovirus200ugQuote
MP488841DULMammalian Cell200ugQuote

Protein Information

SpeciesClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
UniProt IDB8I1T7
Gene NameleuA; Locus:Ccel_3434
Protein Name2-isopropylmalate synthase
Region Expressed1-508
Expression Tag6xHis
Purity>90%
AA SequenceMARTIKIFDTTLRDGEQTPGVNLNLQEKLEIAKQLVRLGVDVIEGGFAIASPGDFESIMT LSRNLKGVTIASLCRSVEKDIDRAWEAVQYAESPRIHTFIATSDIHMKYKLKMTEEEVLE RAVSMVKRAKGYCSNVEFSAEDASRTREEFLYRVVEAVIKAGATTVNIPDTVGYSTPLEF GRLIRNIRNNVPNIDKADISVHCHNDLGLAVANSLAAVENGAVQVECTINGLGERAGNAA LEEIIMGINTRKDYYDITHRIDTTQIYRASRLVSSLTGVNVQPNKAIVGANAFAHESGIH QHGVLSEKTTYEIMTPESVGMGTNRMVLGKLSGRHAFEDRLKEMGYSLSDEEVKTAFAKF KDLADKKKVVTDKDIEALVDENIAVPEIFVIDSFQINSGNKMISTSTVSVRKDEEIITEA ATGDGPVDAAFNAVERATGVNAELVHYRIKAVTEGKDALGEVTVKISNNNSIFMGKGVST DIIEASVKAYLNAINRSISEIGESIISQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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