Request QuoteCatalog Number: xP513872AWCSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513872AWCYeast1mgQuote
EP513872AWCE. coli1mgQuote
BP513872AWCBaculovirus200ugQuote
MP513872AWCMammalian Cell200ugQuote

Protein Information

SpeciesAcidimicrobium ferrooxidans (strain DSM 10331 / JCM 15462 / NBRC 103882 / ICP)
UniProt IDC7M0E4
Gene NameleuA; Locus:Afer_1529
Protein Name2-isopropylmalate synthase
Region Expressed1-516
Expression Tag6xHis
Purity>90%
AA SequenceMDEVGTQERIRIFDTTLRDGEQAPGISLDPLEKLEIAEQLARLGVDIIEAGFPVASQGDF DAVRQIARQVHGPVICGLSRTHVADIERCYEAVRDAEHHRIHVFISTSPSHLEHMLRMSE DQVVEAVRRAIARARELVDDVEFSPQDATRTPLPFLYRVLQVAVDEGASTLNIPDTVGYG IPWDFARMVESVRREVAGSYVISCHCHNDLGLATANSLAAVAAGARQVECCINGIGERAG NAALEEVVMGLAIRSDVIGDVTTGIDTRELARTSRLVSRLTGYPVQYNKAVVGRNAFAHE SGIHQHGVLTDRSTYEVIDAASVGQEAAQIVLGKHSGRHAFQEALARMGIALEGDALNAT FQRFKELADRKVELSEADLEAIVAEELGTTLADRFELVSFRVEAGTGREAVASATVLVDG TPVEASASGNGMVDALGRVLAEATALEARLTGFSVTSVTGGADALGSVAVTVDVGGHEVS GRGVSTDIVEASARALLNALNRAARVREKASNRETP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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