Request QuoteCatalog Number: xP514126GFKSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514126GFKYeast1mgQuote
EP514126GFKE. coli1mgQuote
BP514126GFKBaculovirus200ugQuote
MP514126GFKMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain Y412MC10)
UniProt IDD3EBC1
Gene NameleuA; Locus:GYMC10_4749
Protein Name2-isopropylmalate synthase
Region Expressed1-518
Expression Tag6xHis
Purity>90%
AA SequenceMERNIIVLDTTLRDGEQVPGAKLNVQQKIEFAQQLKRLNVDIIEAGFPASSAGDFQAVQE IARTVGDSVSITALARAVKGDIDAVYESIKLAQNPLIHIVLGTSNIHVEKKFNRSKDAVL QMGVDAVKYAKTLLPQVQYSTEDASRSDFEYLWKTIEAVVKAGATMINVPDTVGYAVPDE FGELIRKINERLKNLNDQVILSVHCHNDLGLATANTLSAVRNGAEKVECTINGLGERAGN TSLEEVVMGLKVRENHFKASTNVRLKELIRTSRLLTHLTGLDVQVNKAITGENAFAHSSG IHQDGLLKDKQVYEIMSPEEVGADSMELILTARSGRHAFKNAVEKLGFETGEGDDFEALF EKFLLLADAKKEVYDHDVFYLVTQHRTHEEVSSHLYELDSFQVVTNDMYPTATVKLKKGS ETFRDSMVGDGPIDALYSAIKALVGLDVQLKDYKINSLSRGKEAIGRVNIRIEYQGKIYS GRAMDTDIIKASALAFLNGINAVLLDAGHDSQAPVSAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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