Request QuoteCatalog Number: xP499733BWLSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP499733BWLYeast1mgQuote
EP499733BWLE. coli1mgQuote
BP499733BWLBaculovirus200ugQuote
MP499733BWLMammalian Cell200ugQuote

Protein Information

SpeciesBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
UniProt IDC0Z9C8
Gene NameleuA; Locus:BBR47_16210
Protein Name2-isopropylmalate synthase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMRTIEIFDTTLRDGEQSPGVNISTNEKVEIALQLEKLGVNRMEAGFAAASPGDQKSVAEV AKRVKNATVVSLARAVKDDMDKAYEALRNAQNASLHVFLATSPIHRQFKLNMSKEEVLAR AVEAVTYAKKYFTEVQFSAEDAARTEIDFLAEVVEAVIKAGATTVNIPDTVGYMTPYQYG NIFRELKKRVPSTDLIRLSCHCHDDLGMAVANSLAAVEGGATQVEGTINGIGERAGNAAL EEVALALETRKDYYQATTKLNLKEIARTSQLVSRLTGMIVPGNKAVVGANAFAHESGIHQ DGVLKEVTTYEIIRPESVGFKSNKLVLGKHSGRHAFKEKLVDLGYHLEQEEVNAAFAAFK VLCDKKKEITDDDILALVDSKMVRGPEAFQLESVQLAYGNISVPTASVRLVRADGSVCEE AACGNGSVDSIYKAIDRATGEEVSLVDYKILSVTHGQDALGEVFVRLQQDDLIVTGRGVS TDVLEASAIAYVRAVNKIMERRGEPTPVSATI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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