Request QuoteCatalog Number: xP501981VEZSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501981VEZYeast1mgQuote
EP501981VEZE. coli1mgQuote
BP501981VEZBaculovirus200ugQuote
MP501981VEZMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain M66-2)
UniProt IDC3LR32
Gene NameleuA; Locus:VCM66_2412
Protein Name2-isopropylmalate synthase
Region Expressed1-516
Expression Tag6xHis
Purity>90%
AA SequenceMNNQVIIFDTTLRDGEQALSASLTVKEKLQIAYALERLGVDIIEAGFPVSSPGDFESVQT IAKNIKNSRVCALSRAVAKDIDAAAEALKVAEAFRIHTFISTSTIHVQDKLRRSYDDVVA MAVKAVKHARQYTDDVEFSCEDAGRTPIDNLCRMVEAAINAGARTINIPDTVGYTVPSEF GGIIQTLFNRVPNIDKAIISVHCHDDLGMSVANSIAAIQAGARQVEGTINGIGERAGNCA LEEIAMIIKTRQELLGVTTGIKHDEISRTSKLVSQLCNMPIQSNKAIVGANAFSHSSGIH QDGMLKNKNTYEIMTPESIGLKNQALNLTSRSGRAAVKSHMDSMGYNENEYNLDALYEDF LKLADRKGQVFDYDLEALMHFSNLREEDDFYKLNYLSVQSGSVMATTSIKLLCGGEEKCE AAVGNGPVDALYQCIYRITGYEIVLDKFDLTAKGEGEDGLGQADIIANYKGRKYHGTGVS TDIVEASGQALLHVINSIHRADQIAQIKQKKSVATV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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