Request QuoteCatalog Number: xP490694DOGSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP490694DOGYeast1mgQuote
EP490694DOGE. coli1mgQuote
BP490694DOGBaculovirus200ugQuote
MP490694DOGMammalian Cell200ugQuote

Protein Information

SpeciesArthrobacter chlorophenolicus (strain A6 / ATCC 700700 / DSM 12829 / JCM 12360)
UniProt IDB8H8Q9
Gene NameleuA; Locus:Achl_1963
Protein Name2-isopropylmalate synthase
Region Expressed1-579
Expression Tag6xHis
Purity>90%
AA SequenceMRNAQKSSGMPFHRYTPFQDQITVEMPDRTWPDKVITKAPRWCAVDLRDGNQALIDPMSP ARKMKMFDLLVRMGYKEIEVGFPSASQTDFDFVRQLIEGNHIPDDVTIQVLTQAREHLIE RTYESLVGAKQAIVHLYNSTSVLQRRVVFNQDEDGILDIALQGARLCRKYEETLADTHIT YEYSPESFTGTELEYAVRVCNAVADVFEASADRQVIINLPATVEMATPNVYADSIEWMSR HLHPREGIILSLHPHNDRGTGVAAAELGYMAGADRIEGCLFGNGERTGNVDLVTLGLNLF VQGIDPMIDFSDIDDVRRTVEYCNQLPVPERSPYGGDLVFTAFSGSHQDAIKKGFEALER DAAAAGKDVADVTWQVPYLPVDPKDLGRSYEAVIRVNSQSGKGGVAYLLKNEHSLDLPRR AQIEFSGVIQRRTDTVGGEVSGAQLWQIFQDEYLPSGKADGQWGRYSLGSVKTETDDDGG MTLHASLTVDGAQVSRTGTGNGPIAALLNILTQDGVDVRVLDYSEHALSEGGNAMAAAYV ECAVGERVLWGVGIDANTSMSSLKAVISAVNRAIRDAQS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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