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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP522173HWBYeast1mgQuote
EP522173HWBE. coli1mgQuote
BP522173HWBBaculovirus200ugQuote
MP522173HWBMammalian Cell200ugQuote

Protein Information

SpeciesHerbaspirillum seropedicae (strain SmR1)
UniProt IDD8J0Q1
Gene NameleuA; Locus:Hsero_0939
Protein Name2-isopropylmalate synthase
Region Expressed1-575
Expression Tag6xHis
Purity>90%
AA SequenceMLTDPSQKYRAIAPVDLPDRRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA RRVIVHFYNAIAPAWREIVFGMSVPQIITLVEDHIALFRRLTAVHPETEWILQYSPETFC MAELEVSLEVCNAAIRAWDAGPRRRMIINLPTTVEVSTPNVFADQIEWMDRRLERREHLI LSVHPHNDRGTAVACAEQAMLAGAQRVEGCLFGNGERSGNVDVVTLALNLYTQGIAPGLD FSDIAALARVAEECTALPIHPRHPYVGDLVFTAFSGSHQDAIAKGFAAQRPDAPWRVPYL PIDPTDLGRTYDSIVRVNSQSGKGGIAFLLQRDHHITMPRRMQVEFSAIVQALADASETE LTSEHLWDVFERTYLAPVQASPDFSDRTSLAPALEQRRFIYRSHCLHPHPDGERIVLELA DTEGNVRTVEGSGNGPIAATVAALGLALRIDSYEERSLGVGVGADAQALAIVEAALPTVP GSRFGVGRHENITTASIMAVLSAASRFAGEEQGKG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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