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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP454902SYZYeast1mgQuote
EP454902SYZE. coli1mgQuote
BP454902SYZBaculovirus200ugQuote
MP454902SYZMammalian Cell200ugQuote

Protein Information

SpeciesShigella boydii serotype 18 (strain CDC 3083-94 / BS512)
UniProt IDB2U281
Gene NameleuA; Locus:SbBS512_E0068
Protein Name
Region Expressed1-523
Expression Tag6xHis
Purity>90%
AA SequenceMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT IARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIE RAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEF AGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIH QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAF LKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKA EAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGL ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQHNENNKETV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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