Request QuoteCatalog Number: xP467765BQMSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP467765BQMYeast1mgQuote
EP467765BQME. coli1mgQuote
BP467765BQMBaculovirus200ugQuote
MP467765BQMMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain AH820)
UniProt IDB7JFY5
Gene NameleuA; Locus:BCAH820_1491
Protein Name
Region Expressed1-506
Expression Tag6xHis
Purity>90%
AA SequenceMKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY ALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQ DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA QDVLEASARAYVHAAGKLKSFIQLVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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