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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP470448SWQYeast1mgQuote
EP470448SWQE. coli1mgQuote
BP470448SWQBaculovirus200ugQuote
MP470448SWQMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella heidelberg (strain SL476)
UniProt IDB4TJ72
Gene NameleuA; Locus:SeHA_C0124
Protein Name
Region Expressed1-523
Expression Tag6xHis
Purity>90%
AA SequenceMSQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT IARTIKNSRVCALARCVEKDIDVAAQALKVADAFRIHTFIATSPMHIATKLRSTLDEVIE RAVYMVKRARNYTDDVEFSCEDAGRTPVDDLARVVEAAINAGARTINIPDTVGYTMPFEF AGIISGLYERVPNIDKAIISVHTHDDLGIAVGNSLAAVHAGARQVEGAMNGIGERAGNCA LEEVIMAIKVRKDIMNVHTNINHHEIWRTSQTVSQICNMPIPANKAIVGSGAFAHSSGIH QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMEEMGYKDTDYNMDHLYDAF LKLADKKGQVFDYDLEALAFINKQQEEPEHFRLDYFSVQSGSSDIATASVKLACGEEIKA EAANGNGPVDAIYQAINRITGYDVELVKYDLNAKGQGKDALGQVDIVVNHHGRRFHGVGL ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQNKENNKETV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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