Request QuoteCatalog Number: xP454504BXTSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP454504BXTYeast1mgQuote
EP454504BXTE. coli1mgQuote
BP454504BXTBaculovirus200ugQuote
MP454504BXTMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia phymatum (strain DSM 17167 / STM815)
UniProt IDB2JDN6
Gene NameleuA; Locus:Bphy_2017
Protein Name
Region Expressed1-516
Expression Tag6xHis
Purity>90%
AA SequenceMADKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIHT IAGMIKDSTICSLARANDKDIQRAADALKPADHFRIHTFIATSPLHMEKKLRMSPEQVLE QAKLAVRFARKFTNDVEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY GNLVKTLRERIPNSDKAVFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT SLEEIVMAVRTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGISLDSESELNLAFQ RFKELADRKAEIFDEDIIAIVTEESAEAQEKEHYKFVSLSQHSETGERPHARIVFSVEGK EVVGEANGNGPVDATLNAIETEVGSGSELLLYSVNAITTGTQAQGEVTVRLSKAGRIVNG VGTDPDIVAASAKAYISALNRLYAGADKLNPQRADI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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