Request QuoteCatalog Number: xP492829SYWSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492829SYWYeast1mgQuote
EP492829SYWE. coli1mgQuote
BP492829SYWBaculovirus200ugQuote
MP492829SYWMammalian Cell200ugQuote

Protein Information

SpeciesShewanella piezotolerans (strain WP3 / JCM 13877)
UniProt IDB8CM39
Gene NameleuA; Locus:swp_2213
Protein Name2-isopropylmalate synthase
Region Expressed1-523
Expression Tag6xHis
Purity>90%
AA SequenceMSDRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQT IARTVKNSRVCALSRALEKDIDAAAQALSVADQFRIHTFISTSTIHVESKLKRSFDQVLE MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIKAGARTINIPDTVGYTVPSEF GGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIH QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMSELGYGDQDYDMDVLYEDF LVLADKKGQVFDYDLEALAFMEAQAEDDDHFKLQQLVVHSDSTEGSATATVKVEVNGETV TEAAIGNGPVDAAYKAVARASGCEIDITSYQLGAKGEGQNALGQVDITASYRNQNFHGVG LATDVVEASVKALVHVMNLTWRADKVADCKQKIQQEKQVLGGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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