Request QuoteCatalog Number: xP456124DZSSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP456124DZSYeast1mgQuote
EP456124DZSE. coli1mgQuote
BP456124DZSBaculovirus200ugQuote
MP456124DZSMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424) )
UniProt IDB2AHM5
Gene NameleuA; Locus:RALTA_B0072
Protein Name
Region Expressed1-569
Expression Tag6xHis
Purity>90%
AA SequenceMLVNPATKYRPAATVDIPDRTWPGRTITRAPRWMSTDLRDGNQALIEPMNPARKLRLFEQ LVKIGLKEIEVAFPAASQTDFDFVRMLIEERRIPDDVTIVVLTQSREDLIRRTVESVRGA ARATVHLYNPIAPAWRRIVFNASRDEIKAVAVSGTRLIKALTDAMPETAWTYEYSPETFS LAELDFSLEVSDAVSAAWQAGPGRPMILNLPTTVECSTPNVFADQIEWMHRRLARRAHIV LSVHPHNDRGTAVAAAELALMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVAPELD FSDIDAVRQCVEHCNQLPVHPRHPYVGDLVFTAFSGSHQDAIRKGFAQQQPDAIWEVPYL PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQVHGLYLPRRLQIEFSRAVQAMTDDTGLE ASADDLYGLFRREYLARETPLRYVSHQLASDATGATAITVQMERDGQPCTVRGTGNGPID AFIDALDLPVRVMDYHEHAMTAGADARAACYVEVRVGDSPTGFGAGIDASLVTASLRAVV SGVNRHLQAGFGARAQATQTASASAATEA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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