Request QuoteCatalog Number: xP454785BXUSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP454785BXUYeast1mgQuote
EP454785BXUE. coli1mgQuote
BP454785BXUBaculovirus200ugQuote
MP454785BXUMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia phytofirmans (strain DSM 17436 / PsJN)
UniProt IDB2SXZ9
Gene NameleuA; Locus:Bphyt_2683
Protein Name
Region Expressed1-572
Expression Tag6xHis
Purity>90%
AA SequenceMLKNPATKYRSFKPVTLTDRQWPSRTITHPPIWMSTDLRDGNQSLFEPMDAQRKMRMFKT LVQIGFKEIEVAFPSASQTDFNFVRELIEGGHIPDDVTIEVLTQARDDLIERTFESLRGV PRAIVHLYNATAPEFRKIVFNLEKSGVKELAQNAARTMKRIAATMPETQFTFQYSPEVFS GTEIEFAKEVCDAVFDVWQPTPEHKAIVNLPATVEMSTPNIYADQIEWMHRNLARRDSLI ISVHPHNDRGTAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGVDPGLD FSNINEVARTAEECTQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQKPDAVWEVPYM PIDPADLGRTYDSVIRVNSQSGKGGIAYLLEQGYGVVLPRRLQVDFSSAVQRFTDDSGQE VTSAQIWELFQQEYVQNTAPIHYVGHSLSEREGRERIKLTVDIHGTRRVLTGEGNGPLDA LMHAIGVPVRIQHYEERALTQGADARAVAVAEMAGADVTGSAFGVGIDANLVTASIRAVI SGVNRAYARVNAQAQENFFDAAMNDAAESVGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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