Request QuoteCatalog Number: xP532921DJMSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP532921DJMYeast1mgQuote
EP532921DJME. coli1mgQuote
BP532921DJMBaculovirus200ugQuote
MP532921DJMMammalian Cell200ugQuote

Protein Information

SpeciesDesulforudis audaxviator (strain MP104C)
UniProt IDB1I1Y1
Gene NameleuA; Locus:Daud_0355
Protein Name2-isopropylmalate synthase
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMSERVYIFDTTLRDGEQSPGVSLNIREKLQIAHQLVKLNVDVIEAGFPFASPGDFKGVQA VAREVRGVSVAGLARANAADIDRAWEALCEAEQPRIHTFIATSDIHLQHKLRMSREAVLE TAVEAVRRAKGYTSDVEFSAEDAFRSDPDYLCRVLAAVIAAGATTVNIPDTVGYAMPDEF GAFIAYIMDHTPGIENVVVSVHCHNDLGLAVANSLAAVRNGARQVECTVNGIGERAGNAA LEEVVMGLFTRREKYGLITGIQCEEIYRTSKLVSTLTGMPVQFNKAIVGKNAFLHESGIH QDGVLKERATYEIICPTMVGITRTNLVLGKHSGRHAFRQRLTELGFELHEEELNRAFVRF KELADSKKQITDEDLEAILDETIRQVPATYELDYLHVFSGTTVKPTAVIGLLIDGQFVQE AACGNGPVDATYKAIEKATGRKYSLVSYVIDAVTGGTDALANVTLKMRDDDHRVYTGRGV SADVLEASARAYVNIMNKIVYDERTRKHE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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