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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP532883LRVYeast1mgQuote
EP532883LRVE. coli1mgQuote
BP532883LRVBaculovirus200ugQuote
MP532883LRVMammalian Cell200ugQuote

Protein Information

SpeciesLysinibacillus sphaericus (strain C3-41)
UniProt IDB1HR98
Gene NameleuA; Locus:Bsph_3386
Protein Name2-isopropylmalate synthase
Region Expressed1-511
Expression Tag6xHis
Purity>90%
AA SequenceMRKIDIFDTTLRDGEQSAGINLNTAEKIEIAKQLERLGVTIIEAGFPASSPGDFDAVHRI AETVKNSVVTGLARCIQKDIDTTWEAIKGAQQPHIHIFLATSPIHMEYKLKKSPEQVIEQ AVEAVKYAKKYFPLVQWSAEDAFRSDREFLVRIMNEVVTAGATTINVPDTVGYASPYEYG ALFKYLLENVKGADKVKFSAHCHDDLGMATANTLAAIENGAAQVEGTINGIGERAGNVAL EEIAVALHIRKDYYQVETGIQLQEIKRTSQLVSKLTNVVIQPNKAIVGRNAFAHESGIHQ DGVLKNPETYEIISPALIGEGEIPLVLGKHSGRAAFRDRAETLGFDLSDEKLNKAFVEFK KLADRKKEITEEDLLTLLTEQQIQLEDVPLFELKMVQVQYGTENIPTATAIVLTPEGLEK TVVATGAGSVEAIFNTLEQLVPNAVHVIDYRVTSVGKGRDALGEAVVNIRYDHVSTTGRN SSQDVLEASAKAYLNAINRHLIKESLRAHPV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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