Request QuoteCatalog Number: xP310612DPXSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP310612DPXYeast1mgQuote
EP310612DPXE. coli1mgQuote
BP310612DPXBaculovirus200ugQuote
MP310612DPXMammalian Cell200ugQuote

Protein Information

SpeciesAzotobacter vinelandii
UniProt IDP96195
Gene NameleuC
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-470
Expression Tag6xHis
Purity>90%
AA SequenceMAGKTLYDKLWDMHEVKRRDDGSSLIYIDRQILHEVTSPQVRGAAPGRANVARGCQHRHP GPQRADHPGSPVGGRRHRRRNLAHPGADPGRELRRVRHRRVQDERPAPGHRPCGRPGAGR HLPGMTVVCGDSHTSTHWCGALAHGIGTSEVEHVLATQCLVAKKMKNMQVRVEGELPLGV SAKDIVLAVIGRIGTAGGTGHALEFAGSAIRGLSMEGRMTLCNMAIEAGARVGMVAVDEK TIDYVKGRPYAPQGADWDKAVAQWRELVSDADAGFDTVVELKAEEIRPQVTWGTSPEMVL PVDERVPDPASESDPVKRDSIVRALKYMGLAANQPIGEIKVDRVFIGSCTNSRIEDLRAA AEVAKGARSPRASSRRWWCRAPGWSRSRRRRRGWTGSSSRPVSSGATGCSMCLAMNPDRL ESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVAGRFVDVRQLLQA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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