Request QuoteCatalog Number: xP530103DOTSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (LEU2)

Recombinant 3-isopropylmalate dehydrogenase (LEU2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP530103DOTYeast1mgQuote
EP530103DOTE. coli1mgQuote
BP530103DOTBaculovirus200ugQuote
MP530103DOTMammalian Cell200ugQuote

Protein Information

SpeciesAshbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
UniProt IDO60027
Gene NameLEU2; Locus:AAL012C
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-372
Expression Tag6xHis
Purity>90%
AA SequenceMAAVKRIVVLPGDHIGREVVEEAVKVLGAVEQSLSDVHFDFQYHLVGGAAIDATGSALPD EALGAAKEADAVLLGAVGGPKWQGGAVRPEQGLLKLRQELGVYANLRPCNFAADSLLELS PLRPEIARDTDIMVVRELLGGSYFGERHEDEGDGVAWDTDKYTVKEVQRIARMAGFLALQ HDPPLPVWSLDKANVLASSRLWRKTVEETFQSEFPNVQLQHQLIDSAAMILVKNPRAFNG VVVTSNMFGDIISDEASVIPGSLGLLPSASLASLPDSKSAFGLYEPCHGSAPDLPAGKAN PIGCILSAAMMLKLSLNMVAAGEAVEQAVQEVLDSGVRTGDLLGSSSTSEVGDAIALAVK EALRRQSAAGLS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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