Request QuoteCatalog Number: xP530756BXESize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP530756BXEYeast1mgQuote
EP530756BXEE. coli1mgQuote
BP530756BXEBaculovirus200ugQuote
MP530756BXEMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
UniProt IDO85064
Gene NameleuB; Locus:BUsg_PL5
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-363
Expression Tag6xHis
Purity>90%
AA SequenceMKKKFRIAVLPGDGIGPEVMREAYKILNILERKFFLKLELREFDIGGIAIDREGIALPEK TLRGCENSDAILFGSVGGKKWDYLPIESRPERASLLPLRKHFNLFSNLRPAKLRVDLKNL SPLRSDIVRDGFDILCIRELTGGIYFGKPKGRFKNKNGEYAFDTEIYYDFEIERIANLAF ELASSRRCKVCSIDKANVLESSVLWREIVKKVSKNYPDVHLSHLYIDNACMQIIKNPTQF DILLCSNIFGDIISDECAMITGSIGMLPSASLNNQKFGLYEPAGGSAPDIEGKNIANPIA QILSLSMLIRYSMNLNEIADKIDLSVHYALKEGYRTLDISDGKNYIKTNEMGDIIAEFLD NGK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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