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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP527505MVZYeast1mgQuote
EP527505MVZE. coli1mgQuote
BP527505MVZBaculovirus200ugQuote
MP527505MVZMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium tuberculosis
UniProt IDO53237
Gene NameleuC; Locus:Rv2988c, MT3066; ORFs:MTV012.02c
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMALQTGEPRTLAEKIWDDHIVVSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAGRRVRR PELTLATEDHNVPTVDIDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGP QLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGR LPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMV APDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTN PGQGVPLAAAVPDPQLMTDDAERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIE DLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGEIFTDAGAQWRQAGCSMCLGM NPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGTLSSPADLN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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