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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500614LPXYeast1mgQuote
EP500614LPXE. coli1mgQuote
BP500614LPXBaculovirus200ugQuote
MP500614LPXMammalian Cell200ugQuote

Protein Information

SpeciesListeria monocytogenes serotype 4b (strain Clip81459)
UniProt IDC1KWT4
Gene NameleuD; Locus:Lm4b_02002
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-193
Expression Tag6xHis
Purity>90%
AA SequenceMEEIKVHIGKTVALMNDNIDTDQIIPKSFLKRIERTGFGEFLFDSWRYLPNRKPNPDFPL NAPDRQEATILITGDNFGCGSSREHAAWALLDYRFRVIIAGSYSDIFYMNCTKNGVLPIV LPREAREKLAKIAADENVTIDLPNQQVISSVGTYPFEIDATWKNKFINGLDDIAITFEHI DAIKAYEQKVDSI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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