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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500613LPXYeast1mgQuote
EP500613LPXE. coli1mgQuote
BP500613LPXBaculovirus200ugQuote
MP500613LPXMammalian Cell200ugQuote

Protein Information

SpeciesListeria monocytogenes serotype 4b (strain Clip81459)
UniProt IDC1KWT3
Gene NameleuC; Locus:Lm4b_02001
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-462
Expression Tag6xHis
Purity>90%
AA SequenceMGKTLFDKLWNRHVIYGKEGEPQLLYVDLHLIHEVTSPQAFEGLRMENRPLRRPDKTFAT MDHNVPTEDIFNIQDLVAKKQIEALQTNCEEFGVTLADMGSDRQGIVHMVGPETGLTQPG KVIVCGDSHTATHGAFGAIGFGIGSSEVEHVFATQTIWQQKPKSMGIEINGNLPKGVYAK DIILHLIATYGVAFGTGYAVEYYGETIRNMSMEERMTICNMAIEGGAKMGMMAPDETTFE YVRGREYAPADMDKAISDWKTLQTDPDAEYDLHIKMDASILEPYVTWGTNPEMGVPFSKA FPEIKDMNYERAYEYMGLKPGQTAEEIELGYVFIGSCTNARLSDLEEAARIVKGNKVKNN IRALVVPGSRQVRNAAESIGLDKIFIEAGFEWREPGCSMCLGMNPDQVPDGVHCASTSNR NFEGRQGKGARTHLVSPAMAAAAAINGHFIDIRKEAVISGGN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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