Request QuoteCatalog Number: xP493694DJNSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP493694DJNYeast1mgQuote
EP493694DJNE. coli1mgQuote
BP493694DJNBaculovirus200ugQuote
MP493694DJNMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949)
UniProt IDB8J4F9
Gene NameleuC; Locus:Ddes_0506
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-420
Expression Tag6xHis
Purity>90%
AA SequenceMAQTLAQKILQAHTDDAVEQDGQIVQCRVSMVLANDITGPLAIKSFYGMGAKKVFDRTKI ALVMDHFTPQKDIDSANQVLISRRFAEEQDIVHYYEGGDCGVEHTLLPEQGLVGPGDLVI GADSHTCTYGGIGAFATGMGSTDIAAGMALGETWLKVPSTIRVNISGDMPRWLRGKDLML MLIGAIGVDGALYKALEFGGSVVDALSVEGRLSMANMAIEAGGKAGLFAVDAKTRTYCAE HKRPGVLENMAADSGAVYERVVDMNVTGKEPVVACPHLPSNVKPVSEVRDTAIQQVVIGS CTNGRISDMRDAAEVLRGRKVDKGVRCIVLPSTPTVWKQCLREGLIEVFMEAGCIVGPST CGPCLGGHMGILGDGERAVATTNRNFRGRMGSLSSEVYLASPLVAAASAVTGYVAGPDQL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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