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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP499503AWJYeast1mgQuote
EP499503AWJE. coli1mgQuote
BP499503AWJBaculovirus200ugQuote
MP499503AWJMammalian Cell200ugQuote

Protein Information

SpeciesAcidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670)
UniProt IDC1F700
Gene NameleuC; Locus:ACP_3469
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMAKTLFEKVWEQHLVAEPANEPALLYIDLHLVHEVTSPQAFDGLRMTGRKLRRPDRTVAT VDHNVPTIAADRLIIKDEIAARQIDALRRNCAEFGVELFDVQSREQGIVHVIGPELGLTK PGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQCLPQGRPKTFRISVEGELPEGVT AKDIVLGIIGRIGTDGATGYVIEYAGSAIRSLSMEGRMTVCNMSIEAGARAGMIAPDETT FAYLKDREYSPKGKAWDEAVAAWSELKTDEGATFDRELVIPAIELTPYVTWGTNPGMVIP ITDEIPGPETAASEADQRGIERALEYMGLKPGTPMEEVAIDVVFIGSCTNSRIEDLRAAA KVVTGYRVNEKVRAMVVPGSHRVKEQAEREGLDRIFREAGFEWREPGCSMCLGMNPDILT PGERCASTSNRNFEGRQGRGGRTHLVSPMMAAAAAITGHFSDIRTWEFKGEEVLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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