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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP509172AWJYeast1mgQuote
EP509172AWJE. coli1mgQuote
BP509172AWJBaculovirus200ugQuote
MP509172AWJMammalian Cell200ugQuote

Protein Information

SpeciesAcidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670)
UniProt IDC1F6Z9
Gene NameleuD; Locus:ACP_3468
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-206
Expression Tag6xHis
Purity>90%
AA SequenceMQPFRTLHSTVTPLDRVNVDTDQIIPKQFLKRIERTGYGEFLFFDWRQDPAFELNQPRYK GSQILVANKNFGCGSSREHAAWALGDFGFRCVISSSFADIFHSNAGKNGILLVKLSEGDV NTLLERAQKTQGYSLTVSLEEQTVKDGQGFSDSFEIDAFRRYCLLEGLDDIGLTMRYQDK LDAFEKEHDGKFWLAPRGGLAASRTS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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