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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492500DJIYeast1mgQuote
EP492500DJIE. coli1mgQuote
BP492500DJIBaculovirus200ugQuote
MP492500DJIMammalian Cell200ugQuote

Protein Information

SpeciesDesulfatibacillum alkenivorans (strain AK-01)
UniProt IDB8FMM4
Gene NameleuC; Locus:Dalk_0181
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-419
Expression Tag6xHis
Purity>90%
AA SequenceMGMTIAEKILAAHAGQEEASPGDLINAKVDIALGNDITAPIAIKLFRQSGAAKVFDKDRV VLVPDHFTPTKDINSAMQVKMVRDFAREQELSHWYEGGDSGVEHALLPEKGIVGPGDLVI GADSHTCTYGGLGAFATGVGSTDLAAAMITGEVWLKVPESIKFVYKGKLRPHVEGKDLIL HTIGDIGVDGALYMAMEFTGEVIDALPMAERLTMANMAIEAGGKAGIIAPDAKTKEYADG RVIRKPVFYASDPDAKYAKVIEYDVTDLEPQVAFPHLPENTRPISQVGEVPIHQVIIGSC TNGRIEDMRSAARILTGNKADKNVRLIIIPATPQIYRQAMEEGLFDTFLSAGAVISPPTC GPCLGGHMGILAAGERAVATTNRNFVGRMGHVESEVYLANPAVAAASAIAGKIAGPDDI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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