Request QuoteCatalog Number: xP490521HTDSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP490521HTDYeast1mgQuote
EP490521HTDE. coli1mgQuote
BP490521HTDBaculovirus200ugQuote
MP490521HTDMammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus parasuis serovar 5 (strain SH0165)
UniProt IDB8F3W8
Gene NameleuC; Locus:HAPS_0336
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-468
Expression Tag6xHis
Purity>90%
AA SequenceMKKTLYEKLFDAHVVHEAEGETPLIYINRHLVHEVTSPQAFDGLRAMGREVRQPTKTVAT MDHNVPTDSRDLGGSGEMGRIQMVELAKNTEQFGVTLYDINHINQGIVHVMGPEQGLTLP GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTVKQARAKKMKIEVRGKVREGITA KDIVLAIIGKTTMAGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIELGAKSGLIAPDETTF AYLKDRPSAPKGKDWDDAVAYWKTLHTDEGAEFDTVVTLEASEIEPQVTWGTNPGHVIGI NETIPNPADMADPIERQSAEKALAYMGLPHSIKLTDVAIDKVFIGSCTNSRIEDLRAAAA VAKGRKVADGVQALVVPGSGLVREQAEKEGLDKIFIEAGFEWRQPGCSMCLAMNNDRLEP GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVHGKFVDIRNVELH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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