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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503018FPFYeast1mgQuote
EP503018FPFE. coli1mgQuote
BP503018FPFBaculovirus200ugQuote
MP503018FPFMammalian Cell200ugQuote

Protein Information

SpeciesSulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)
UniProt IDC3N992
Gene NameleuD; Locus:YG5714_0261
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-165
Expression Tag6xHis
Purity>90%
AA SequenceMIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKV FGMGSSREQAAIALKAAGVKAVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKI DVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKKMQTVNRN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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