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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP489405DQPYeast1mgQuote
EP489405DQPE. coli1mgQuote
BP489405DQPBaculovirus200ugQuote
MP489405DQPMammalian Cell200ugQuote

Protein Information

SpeciesCaulobacter crescentus (strain NA1000 / CB15N)
UniProt IDB8GY66
Gene NameleuC; Locus:CCNA_00196
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-479
Expression Tag6xHis
Purity>90%
AA SequenceMSGKTLYDKIWDAHVVSEAGGEAILYIDLHLIHEVTTPQAFAGLRAAGRKVRRPDRTLAV ADHNIPTEGQALGVDAVADEEARLQLKTLARNVADNGIEFFPMGDIRNGIVHVVGPEQGR TQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLRQKKAKNMLVRVDGQLPPG VTGKDVALAVIGEIGTAGGTGYVIEFAGEAIAGLSMEGRMTLCNLTIEGGAKAGLVAPDD KTFAYIQGKPAAPKGAAWDMALSHWKTFFTDEDAVFDRTVVIDGSALVPMVTWGTSPEDV IPVTGNVPDPESFATPDKRAAAHRALDYMGLKAGQPISEARIDRVFIGSCTNSRIEDMRA AAAVVQEAFLHGRLVAPHVKAMVVPGSGLVKEQAEEEGLDAIFKAAGFDWREPGCSMCLA MNPDKLAPQERCASTSNRNFEGRQGRAGRTHLVSPAMAAAAAIAGHLVDVRTLFSETAQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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