Request QuoteCatalog Number: xP488734TNXSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP488734TNXYeast1mgQuote
EP488734TNXE. coli1mgQuote
BP488734TNXBaculovirus200ugQuote
MP488734TNXMammalian Cell200ugQuote

Protein Information

SpeciesThioalkalivibrio sp. (strain HL-EbGR7)
UniProt IDB8GQD6
Gene NameleuC; Locus:Tgr7_1245
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-470
Expression Tag6xHis
Purity>90%
AA SequenceMTGKTLYDKLWDAHVVRTEPDGTALLYIDRHLVHEVTSPQAFEGLRLAGRKPWRTDTVLA VPDHNVPTTHRDQGISDPVSRIQVETLDANVHEFGITFFPMNDVRQGIVHVIGPEQGATL PGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLLQKKMKNMLVSVDGRVGPGVT AKDIVLAIIGEIGTAGGTGYAIEFGGEAIRDLSMEGRMTVCNMAIEAGARAGMVAVDETT IEYVKGRTYAPTGEQWESAVAAWRELNSDADARFDRVVRLDATAIAPQVSWGTSPEMVVP VTGRVPDPAEATDPVKASGMKRALEYMGLAANTPIEQIAIDKVFIGSCTNSRIEDLRAAA AVVRGRKVAGNVKLAMVVPGSGLVKQQAEQEGLDQVFLEAGFEWREPGCSMCLAMNADRL EPGERCASTSNRNFEGRQGQGGRTHLVSPAMAAAAAVTGHFTDVRQLAQS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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