Request QuoteCatalog Number: xP507883DJKSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP507883DJKYeast1mgQuote
EP507883DJKE. coli1mgQuote
BP507883DJKBaculovirus200ugQuote
MP507883DJKMammalian Cell200ugQuote

Protein Information

SpeciesDesulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
UniProt IDC0QDY4
Gene NameleuD; Locus:HRM2_43490
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-214
Expression Tag6xHis
Purity>90%
AA SequenceMKPFTTHTGVIATLNRSNVDTDAIIPKQFLKSIKRTGYGPSAFYDWRYTADGRPDPNFEL NHPRFEGRSILVTRNNFGCGSSREHAVWALVQDGYRVIIAPWKEIGEKRLPAFADIFLSN TTKNGMLCIELSETIIDAIFEQTASQPGLQATVDLTVQQLTIHGRIPATYPFQIEEGVRE QLINGLDEIALSLKHEADIAAFEARRPTWMDGRD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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