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Recombinant 3-isopropylmalate dehydrogenase (leuB)

Recombinant 3-isopropylmalate dehydrogenase (leuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP507400MVIYeast1mgQuote
EP507400MVIE. coli1mgQuote
BP507400MVIBaculovirus200ugQuote
MP507400MVIMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019)
UniProt IDC1AGB0
Gene NameleuB; Locus:JTY_3011
Protein Name3-isopropylmalate dehydrogenase
Region Expressed1-336
Expression Tag6xHis
Purity>90%
AA SequenceMKLAIIAGDGIGPEVTAEAVKVLDAVVPGVQKTSYDLGARRFHATGEVLPDSVVAELRNH DAILLGAIGDPSVPSGVLERGLLLRLRFELDHHINLRPARLYPGVASPLSGNPGIDFVVV REGTEGPYTGNGGAIRVGTPNEVATEVSVNTAFGVRRVVADAFERARRRRKHLTLVHKTN VLTLAGGLWLRTVDEVGECYPDVEVAYQHVDAATIHMITDPGRFDVIVTDNLFGDIITDL AAAVCGGIGLAASGNIDATRANPSMFEPVHGSAPDIAGQGIADPTAAIMSVALLLSHLGE HDAAARVDRAVEAHLATRGSERLATSDVGERIAAAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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