Request QuoteCatalog Number: xP471691AZMSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP471691AZMYeast1mgQuote
EP471691AZME. coli1mgQuote
BP471691AZMBaculovirus200ugQuote
MP471691AZMMammalian Cell200ugQuote

Protein Information

SpeciesAliivibrio salmonicida (strain LFI1238) (Vibrio salmonicida (strain LFI1238) )
UniProt IDB6ELJ8
Gene NameleuC; Locus:VSAL_I0375
Protein Name
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMSNAKTLYEKVYDAHVAVAAKGETPILYIDRHLVHEVTSPQAFDGLREKGRNVRQVGKTF ATMDHNVSTQTKDINASGEMARIQMETLSKNCEEFGVTLYDLNHKYQGIVHVMGPELGIT LPGMTIVCGDSHTATHGAFGSLAFGIGTSEVEHVLATQTLKQARAKTMKIEVKGKVTEGI TAKDIVLAIIGKTTAAGGTGYVVEFCGEAITDLTMEGRMTVCNMAIELGAKAGLIAPDAT TFDYITGRKFSPQGEDLTAAIEYWSSLKTDDDAKFDAVVSLDASEIKPQVTWGTNPGQVI AIDQPIPAPESFSDPVEKVSAEKALAYMGLEAGKSLTDYNVDKVFVGSCTNSRIEDIRAA AAVAKGNKVAAHVQALIVPGSEQVKAQAEKEGLDIIFKEAGFEWRLPGCSMCLAMNNDRL GPQERCASTSNRNFEGRQGRDGRTHLVSPAMAAAAAIAGHFVDIRTITEKA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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