Request QuoteCatalog Number: xP465523EUPSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP465523EUPYeast1mgQuote
EP465523EUPE. coli1mgQuote
BP465523EUPBaculovirus200ugQuote
MP465523EUPMammalian Cell200ugQuote

Protein Information

SpeciesPhenylobacterium zucineum (strain HLK1)
UniProt IDB4RCC2
Gene NameleuC; Locus:PHZ_c0107
Protein Name
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMPGKTLYDKIWDAHVVAEQDGEAILYIDLHLIHEVTTPQAFAGLRVAGRPVRRPDRTLAV ADHNVPTEGQGLGIDAVADEEARLQLQTLAKNVAEHGIEFFSMGDVRNGIVHVVGPEQGR TQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLRQKKAKNMRVRIEGEPAPG VGAKDFALAVIGEIGTAGGTGYVIEYAGSAVRALSMEGRMTLCNLTIEGGAKAGLIAPDE MTYAYLQGRPAAPKGGAWEMALDYWKGFHSDEDAVFDREIVLDAAKIAPTVTWGTSPEDV VAVTGLVPSPDSFGTPDKRASAARALEYMGLTAGQPITEAKVDVVFIGSCTNSRIEDLRA AAAIVAKALEGGRKVAPGVRAMVVPGSGLVREQAEAEGLDEVFKAAGFDWREPGCSMCLG MNPDRLAPGERCASTSNRNFEGRQGRGGRTHLMSPAMAAAAAIAGHIADVREFL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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