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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP464871BTAYeast1mgQuote
EP464871BTAE. coli1mgQuote
BP464871BTABaculovirus200ugQuote
MP464871BTAMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium longum (strain DJO10A)
UniProt IDB3DPI2
Gene NameleuC; Locus:BLD_1596
Protein Name
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMGTTLAEKVWADHLVRKGSDGAPDLLYIDLMLMHEVTSPQAFEGLRLAGRKPRHVDQLIA TEDHNTPTVDIDRPNPDETSALQLTTLEKNCKEFGVRLHPLGDADQGIVHAFAPILGLTQ PGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQTLSLKPFKTMAVNVNGKLPADAT AKDIILAIIAKIGTGGGQGYVIEYRGEAIRNLTMDERMTVCNMSIEAGARAGMIAPDETT FEYLKGRPHAPEGELWDQAVAYWKTLKTDDDAVFDKVVDIDATKLGPYVTWGTNPGQGLP ITASVPEPGKIADATKRAAAERAITYMGLKPGMPIKDIAVDTVFIGSCTNGRIDDLRQAA AIMKGHRKAENIHRVLVVPASSRVRLQAEKEGLDKVFKDFGAEWRNAGCSMCLGMNPDKL VPNERSISTSNRNFEGRQGKGSRTHLASPAVAAATAIRGTISSPADL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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